Hotspot

psbD-trnT-GGU

LSC · 24507-25742 · 1236 bp

Back to Terniopsis

Pi 0.0429

MarkerSeek score 82.60

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1236
variable_sites: 98
pi: 0.0429
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	ACGTAACCAGTCATCCATAA	GTGGTAGAGTAACGCCATGG	40.0	55.0	53.8	58.1	1346-1378 bp	1.0	54.2
psbD-trnT-GGU_p2	ACGTAACCAGTCATCCATAA	TCAGTGGTAGAGTAACGCCA	40.0	50.0	53.8	58.1	1349-1381 bp	1.0	54.0
psbD-trnT-GGU_p3	ACGTAACCAGTCATCCATAA	GGCGTAAGTCATCGGTTCAA	40.0	50.0	53.8	58.3	1323-1355 bp	1.0	52.6
psbD-trnT-GGU_p4	ACGTAACCAGTCATCCATAAT	GTGGTAGAGTAACGCCATGG	38.1	55.0	54.4	58.1	1346-1378 bp	1.0	51.4
psbD-trnT-GGU_p5	ACGTAACCAGTCATCCATAAT	TCAGTGGTAGAGTAACGCCA	38.1	50.0	54.4	58.1	1349-1381 bp	1.0	51.2