Hotspot

trnV-UAC-ndhC

LSC · 7542-8231 · 690 bp

Back to Terniopsis

Pi 0.0594

MarkerSeek score 82.80

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 78
pi: 0.0594
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	CCGAGAAGGTCTACGGTTCA	AGGGGTGATAAAATTCCGGA	55.0	45.0	58.8	56.2	827-868 bp	1.0	66.8
trnV-UAC-ndhC_p2	CGAGAAGGTCTACGGTTCAA	AGGGGTGATAAAATTCCGGA	50.0	45.0	57.0	56.2	826-867 bp	1.0	65.4
trnV-UAC-ndhC_p3	CCGAGAAGGTCTACGGTTCA	AAGGGGTGATAAAATTCCGGA	55.0	42.9	58.8	57.0	828-869 bp	1.0	65.0
trnV-UAC-ndhC_p4	CCGAGAAGGTCTACGGTTCA	AGGGGTGATAAAATTCCGGAA	55.0	42.9	58.8	57.0	827-868 bp	1.0	65.0
trnV-UAC-ndhC_p5	CGAGAAGGTCTACGGTTCAA	AGGGGTGATAAAATTCCGGAA	50.0	42.9	57.0	57.0	826-867 bp	1.0	63.6