Hotspot

trnM-CAU-trnV-UAC

LSC · 6592-6780 · 189 bp

Back to Terniopsis

Pi 0.0607

MarkerSeek score 81.80

Primer available yes

Label trnM-CAU-trnV-UAC

Feature Metadata

label_name: trnM-CAU-trnV-UAC
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 20
pi: 0.0607
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnV-UAC_p1	TTGAACCAATGACTCTCGCC	AACGAGTTGCTCTACCGAAC	50.0	50.0	58.2	57.9	277-281 bp	1.0	85.1
trnM-CAU-trnV-UAC_p2	TCTCGCCGTATGAAAGCAAT	AACGAGTTGCTCTACCGAAC	45.0	50.0	57.7	57.9	264-268 bp	1.0	84.6
trnM-CAU-trnV-UAC_p3	CTCTCGCCGTATGAAAGCAA	AACGAGTTGCTCTACCGAAC	50.0	50.0	58.4	57.9	265-269 bp	1.0	84.1
trnM-CAU-trnV-UAC_p4	CAATGACTCTCGCCGTATGA	AACGAGTTGCTCTACCGAAC	50.0	50.0	57.5	57.9	271-275 bp	1.0	82.9
trnM-CAU-trnV-UAC_p5	CCAATGACTCTCGCCGTATG	AACGAGTTGCTCTACCGAAC	55.0	50.0	58.5	57.9	272-276 bp	1.0	82.7