Hotspot

rps2

LSC · 23359-24069 · 711 bp

Back to Tephroseris

Pi 0.00281

MarkerSeek score 60.40

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TTTTTCAAAAGGGGGAGGCA	TGAGATTCAGAAATAGTAACCGA	45.0	34.8	57.8	54.9	821 bp	1.0	40.2
rps2_p2	TTTTTCAAAAGGGGGAGGCA	AAATCTATGAAGGTTCCGAAA	45.0	33.3	57.8	52.8	792 bp	1.0	40.2
rps2_p3	TTTTCAAAAGGGGGAGGCAG	TGAGATTCAGAAATAGTAACCGA	50.0	34.8	58.3	54.9	820 bp	1.0	40.2
rps2_p4	TTTTCAAAAGGGGGAGGCAG	AAATCTATGAAGGTTCCGAAA	50.0	33.3	58.3	52.8	791 bp	1.0	40.2
rps2_p5	TTTTTCAAAAGGGGGAGGCA	TCAATAAATCTATGAAGGTTCCGA	45.0	33.3	57.8	55.7	797 bp	1.0	40.2