Hotspot

rpl32-trnL-UAG

SSC · 111012-111822 · 811 bp

Back to Tephroseris

Pi 0.00377
MarkerSeek score 62.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
811
variable_sites
3
pi
0.00377
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 913-928 bp 1.0 80.5
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 914-929 bp 1.0 80.5
rpl32-trnL-UAG_p3 AAAGGATATTGGGCAGCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 924-939 bp 1.0 80.4
rpl32-trnL-UAG_p4 AAAGGATATTGGGCAGCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 925-940 bp 1.0 80.4
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCTTTGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 923-938 bp 1.0 79.6