Hotspot

petL-petG

LSC · 64555-64707 · 153 bp

Back to Tephroseris

Pi 0.0131

MarkerSeek score 60.00

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 2
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGGTCTGAGCAAGATACGAC	TCAAAGGTCCAACTGATCGC	50.0	50.0	57.1	58.2	295 bp	1.0	71.5
petL-petG_p2	TTGGTCTGAGCAAGATACGAC	TCAAAGGTCCAACTGATCGC	47.6	50.0	57.8	58.2	296 bp	1.0	69.2
petL-petG_p3	GGTCTGAGCAAGATACGACT	TCAAAGGTCCAACTGATCGC	50.0	50.0	56.8	58.2	294 bp	1.0	69.1
petL-petG_p4	TCGGTTTTCTACTAACGGCT	TCAAAGGTCCAACTGATCGC	45.0	50.0	56.6	58.2	341 bp	1.0	67.7
petL-petG_p5	GGTCTGAGCAAGATACGACTT	TCAAAGGTCCAACTGATCGC	47.6	50.0	57.5	58.2	294 bp	1.0	66.9