Hotspot

accD-psaI

LSC · 57687-58364 · 678 bp

Back to Tephroseris

Pi 0.00442

MarkerSeek score 61.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 3
pi: 0.00442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	853-858 bp	1.0	66.3
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	852-857 bp	1.0	66.2
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.2	58.0	854-859 bp	1.0	66.2
accD-psaI_p4	TCCAGAAGGGCTTATTCGAT	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.0	58.0	853-858 bp	1.0	64.3
accD-psaI_p5	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	843-848 bp	1.0	64.2