Hotspot

atpA-trnR-UCU

LSC · 29605-29714 · 110 bp

Back to Tephroseris

Pi 0.0182

MarkerSeek score 63.00

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 110
variable_sites: 2
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	AAGACATTCACCGAGGAAGC	GCACTGAAAAGCGTCCATTG	50.0	50.0	57.9	58.3	282 bp	1.0	77.3
atpA-trnR-UCU_p2	TACTTCAGGAACAAGCAGCC	GCACTGAAAAGCGTCCATTG	50.0	50.0	57.8	58.3	215 bp	1.0	76.8
atpA-trnR-UCU_p3	AAGACATTCACCGAGGAAGC	AGCACTGAAAAGCGTCCATT	50.0	45.0	57.9	58.4	283 bp	1.0	76.6
atpA-trnR-UCU_p4	CAGGAACAGAGGGAACGTTT	GCACTGAAAAGCGTCCATTG	50.0	50.0	57.7	58.3	237 bp	1.0	76.1
atpA-trnR-UCU_p5	AAGACATTCACCGAGGAAGC	TAGCACTGAAAAGCGTCCAT	50.0	45.0	57.9	57.5	284 bp	1.0	76.0