Hotspot

rps16-trnQ-UUG

LSC · 6328-7290 · 963 bp

Back to Tephroseris

Pi 0.00105
MarkerSeek score 60.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
963
variable_sites
1
pi
0.00105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1036-1053 bp 1.0 79.1
rps16-trnQ-UUG_p2 GTGGTAAGAATCCACGTCGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1068-1085 bp 1.0 77.3
rps16-trnQ-UUG_p3 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1051-1068 bp 1.0 77.3
rps16-trnQ-UUG_p4 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1050-1067 bp 1.0 77.3
rps16-trnQ-UUG_p5 GTCGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1053-1070 bp 1.0 77.3