Hotspot

ndhB

Genome · 1-1223 · 1223 bp

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Pi 0.0823

MarkerSeek score 81.00

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 1223
variable_sites: 99
pi: 0.0823
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	TGGGGAAAAAGAGGGAGCTA	GACCGCTTCTGAAAATTGGC	50.0	50.0	57.9	58.0	916 bp	0.5	59.4
ndhB_p2	TGGGGAAAAAGAGGGAGCTA	TATTAACTGCAGGTTCGGGC	50.0	50.0	57.9	58.0	773 bp	0.5	59.3
ndhB_p3	CCAACAGGTGGGGAAAAAGA	GACCGCTTCTGAAAATTGGC	50.0	50.0	57.9	58.0	924 bp	0.5	59.2
ndhB_p4	CCAACAGGTGGGGAAAAAGA	TATTAACTGCAGGTTCGGGC	50.0	50.0	57.9	58.0	781 bp	0.5	59.1
ndhB_p5	TGGGGAAAAAGAGGGAGCTA	TTTCTATTGATGGCTGGGGC	50.0	50.0	57.9	57.9	624 bp	0.5	59.0