Hotspot

rpl21-ndhD

Genome · 108805-109196 · 392 bp

Back to Tectaria

Pi 0.164

MarkerSeek score 75.40

Primer available yes

Label rpl21-ndhD

Feature Metadata

label_name: rpl21-ndhD
feature_type: igs
region: Genome
length_bp: 392
variable_sites: 64
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl21-ndhD_p1	AAGTGTGACTCGATTCGTCG	GATACGGGCTGATTCGGATC	50.0	55.0	58.0	58.0	1190 bp	0.5	59.7
rpl21-ndhD_p2	AAGTGTGACTCGATTCGTCG	CAGGAATGAGCGGTTTCGTA	50.0	50.0	58.0	58.0	797 bp	0.5	59.7
rpl21-ndhD_p3	GTGACTCGATTCGTCGTTGA	GATACGGGCTGATTCGGATC	50.0	55.0	58.0	58.0	1186 bp	0.5	59.7
rpl21-ndhD_p4	GTGACTCGATTCGTCGTTGA	CAGGAATGAGCGGTTTCGTA	50.0	50.0	58.0	58.0	793 bp	0.5	59.6
rpl21-ndhD_p5	AAGTGTGACTCGATTCGTCG	GCTTCCATGAGTCAGCGTAA	50.0	50.0	58.0	58.0	1083 bp	0.5	59.6