Hotspot

rrn16-rps12

Genome · 92982-94887 · 1906 bp

Back to Tectaria

Pi 0.105

MarkerSeek score 93.30

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: Genome
length_bp: 1906
variable_sites: 199
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 93.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	TCCGACTTGCATGTGTTAGG	GAACTCTCGCGACTTTACGT	50.0	50.0	57.9	58.0	2025 bp	0.5	58.9
rrn16-rps12_p2	TCCGACTTGCATGTGTTAGG	CTCTCGCGACTTTACGTAGG	50.0	55.0	57.9	57.9	2022 bp	0.5	58.5
rrn16-rps12_p3	TCCGACTTGCATGTGTTAGG	AGCCGTTACCTCAAACTTGG	50.0	50.0	57.9	58.1	2054 bp	0.5	58.4
rrn16-rps12_p4	TCCGACTTGCATGTGTTAGG	GCCGTTACCTCAAACTTGGA	50.0	50.0	57.9	58.1	2053 bp	0.5	58.3
rrn16-rps12_p5	TCCGACTTGCATGTGTTAGG	GGTTAATCGAACTCTCGCGA	50.0	50.0	57.9	57.8	2033 bp	0.5	57.8