Hotspot

petA-psbJ

Genome · 59969-60705 · 737 bp

Back to Tectaria

Pi 0.0954

MarkerSeek score 81.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 737
variable_sites: 69
pi: 0.0954
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AAGGAAGGGGTGGATACGAA	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	1120-1239 bp	1.0	85.9
petA-psbJ_p2	AGGAAGGGGTGGATACGAAA	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	1119-1238 bp	1.0	85.9
petA-psbJ_p3	TAATCGCATTCCAGCCGAAA	GCCGGTACACTTGTGATAGG	45.0	55.0	58.0	58.1	1419-1538 bp	1.0	84.7
petA-psbJ_p4	TAATCGCATTCCAGCCGAAA	GTTCATGTTTCGAGTCGGGA	45.0	50.0	58.0	57.9	1532-1651 bp	1.0	84.5
petA-psbJ_p5	TAATCGCATTCCAGCCGAAA	TTCATGTTTCGAGTCGGGAC	45.0	50.0	58.0	57.9	1531-1650 bp	1.0	84.5