Hotspot

trnT-GGU-trnfM-CAU

Genome · 35736-36174 · 439 bp

Back to Tectaria

Pi 0.0963

MarkerSeek score 79.90

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: Genome
length_bp: 439
variable_sites: 42
pi: 0.0963
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	TTAGCCCCTTGTCGGATTTG	CAGGTTCAAATCCTGTCCCC	50.0	55.0	57.9	58.2	537-588 bp	1.0	84.1
trnT-GGU-trnfM-CAU_p2	TTAGCCCCTTGTCGGATTTG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.9	57.8	568-619 bp	1.0	83.3
trnT-GGU-trnfM-CAU_p3	TTAGCCCCTTGTCGGATTTG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.9	57.8	567-618 bp	1.0	83.3
trnT-GGU-trnfM-CAU_p4	ACGACTTACGCCTTACCATG	CAGGTTCAAATCCTGTCCCC	50.0	55.0	57.7	58.2	512-563 bp	1.0	83.1
trnT-GGU-trnfM-CAU_p5	ACGACTTACGCCTTACCATG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.7	57.8	542-593 bp	1.0	82.2