Hotspot

psbD-trnT-GGU

Genome · 34238-35663 · 1426 bp

Back to Tectaria

Pi 0.0819

MarkerSeek score 79.00

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1426
variable_sites: 116
pi: 0.0819
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TTTTCCGATGGCAATGGTCA	TCTGATTTGAGGCCCCCTTA	45.0	50.0	58.1	58.0	1530-1551 bp	1.0	85.7
psbD-trnT-GGU_p2	TTTCCGATGGCAATGGTCAT	TCTGATTTGAGGCCCCCTTA	45.0	50.0	57.9	58.0	1529-1550 bp	1.0	85.2
psbD-trnT-GGU_p3	TTTTCCGATGGCAATGGTCA	ATTTCTGATTTGAGGCCCCC	45.0	50.0	58.1	57.8	1533-1554 bp	1.0	84.5
psbD-trnT-GGU_p4	TTTCCGATGGCAATGGTCAT	ATTTCTGATTTGAGGCCCCC	45.0	50.0	57.9	57.8	1532-1553 bp	1.0	84.1
psbD-trnT-GGU_p5	TTTTCCGATGGCAATGGTCA	AACTCAGGGGTAGAGTGACG	45.0	55.0	58.1	58.5	1511-1532 bp	1.0	82.3