Hotspot

rpoB-trnD-GUC

Genome · 26739-27392 · 654 bp

Back to Tectaria

Pi 0.0896

MarkerSeek score 79.80

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: Genome
length_bp: 654
variable_sites: 58
pi: 0.0896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	GTTCTGGTAGTACGGGCAT	TTATCGGGATTGACGGGTCT	52.6	50.0	56.5	58.2	746-751 bp	1.0	65.5
rpoB-trnD-GUC_p2	GTTCTGGTAGTACGGGCAT	GTTTATCGGGATTGACGGGT	52.6	50.0	56.5	57.7	748-753 bp	1.0	64.7
rpoB-trnD-GUC_p3	GTTCTGGTAGTACGGGCAT	TTTATCGGGATTGACGGGTC	52.6	50.0	56.5	57.4	747-752 bp	1.0	62.4
rpoB-trnD-GUC_p4	GTTCTGGTAGTACGGGCAT	AGTTTATCGGGATTGACGGG	52.6	50.0	56.5	57.4	749-754 bp	1.0	62.3
rpoB-trnD-GUC_p5	GTTCTGGTAGTACGGGCAT	TATCGGGATTGACGGGTCTC	52.6	55.0	56.5	58.7	745-750 bp	1.0	61.9