Hotspot

atpH-atpI

Genome · 13627-14416 · 790 bp

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Pi 0.105

MarkerSeek score 79.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 790
variable_sites: 64
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGCAATAACAGAAGCAGC	GCTGCAGCTTATATTGGGGA	50.0	50.0	58.1	57.7	690-870 bp	1.0	87.6
atpH-atpI_p2	AGCAATAACAGAAGCAGCAGA	GCTGCAGCTTATATTGGGGA	42.9	50.0	57.9	57.7	688-868 bp	1.0	79.9
atpH-atpI_p3	CAGCAATAACAGAAGCAGCAG	GCTGCAGCTTATATTGGGGA	47.6	50.0	57.9	57.7	689-869 bp	1.0	79.3
atpH-atpI_p4	GCAGCAATAACAGAAGCAGC	GCTGCAGCTTATATTGGGGAA	50.0	47.6	58.1	58.4	690-870 bp	1.0	78.5
atpH-atpI_p5	CAGCAATAACAGAAGCAGCA	GCTGCAGCTTATATTGGGGA	45.0	50.0	56.7	57.7	689-869 bp	1.0	78.1