Hotspot

trnS-GCU-ycf12

Genome · 7992-8554 · 563 bp

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Pi 0.103
MarkerSeek score 80.10
Primer available yes
Label trnS-GCU-ycf12

Feature Metadata

label_name
trnS-GCU-ycf12
feature_type
igs
region
Genome
length_bp
563
variable_sites
58
pi
0.103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-ycf12_p1 GCAATCCGACGCTTTAAACC CAAGTTGCGCAATTGTTTCT 50.0 40.0 58.1 56.1 627-660 bp 1.0 70.8
trnS-GCU-ycf12_p2 CAATCCGACGCTTTAAACCC CAAGTTGCGCAATTGTTTCT 50.0 40.0 57.5 56.1 626-659 bp 1.0 67.4
trnS-GCU-ycf12_p3 AATCCGACGCTTTAAACCCC CAAGTTGCGCAATTGTTTCT 50.0 40.0 58.5 56.1 625-658 bp 1.0 67.3
trnS-GCU-ycf12_p4 CTTTAAACCCCTCGGCCATC CAAGTTGCGCAATTGTTTCT 55.0 40.0 58.6 56.1 616-649 bp 1.0 66.9
trnS-GCU-ycf12_p5 GCAATCCGACGCTTTAAACC AGTTGCGCAATTGTTTCTAGA 50.0 38.1 58.1 56.5 625-658 bp 1.0 66.1