Hotspot

psbK-psbI

Genome · 7191-7718 · 528 bp

Back to Tectaria

Pi 0.126

MarkerSeek score 83.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 528
variable_sites: 66
pi: 0.126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	ACCGGTGTTTTTCCTTCTCC	CCGAAGACGAAGAGAGATACG	50.0	52.4	58.0	57.8	651-662 bp	1.0	78.2
psbK-psbI_p2	CCGCAGTTAGCTTCCGATAA	CCGAAGACGAAGAGAGATACG	50.0	52.4	57.8	57.8	613-624 bp	1.0	76.7
psbK-psbI_p3	CCGGTGTTTTTCCTTCTCCT	CCGAAGACGAAGAGAGATACG	50.0	52.4	57.7	57.8	650-661 bp	1.0	76.3
psbK-psbI_p4	TAGCTTTTGTTTGGCAAGCC	CCGAAGACGAAGAGAGATACG	45.0	52.4	57.5	57.8	631-642 bp	1.0	74.2
psbK-psbI_p5	ACCGGTGTTTTTCCTTCTCC	CGAAGACGAAGAGAGATACGA	50.0	47.6	58.0	56.8	650-661 bp	1.0	70.6