Hotspot

ycf2

Genome · 73952-80734 · 6783 bp

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Pi 0.00059

MarkerSeek score 52.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6783
variable_sites: 4
pi: 0.00059
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CCCAGCATTCATCAAGTTGG	TCCAAACCAACCCCAATGTT	50.0	45.0	57.0	57.8	233 bp	1.0	71.3
ycf2_p2	CCCAGCATTCATCAAGTTGG	TTCCAAACCAACCCCAATGT	50.0	45.0	57.0	57.8	234 bp	1.0	71.3
ycf2_p3	CCCAGCATTCATCAAGTTGG	AACCCCAATGTTGAGTAGCC	50.0	50.0	57.0	57.8	225 bp	1.0	71.0
ycf2_p4	CCCAGCATTCATCAAGTTGG	CCAAACCAACCCCAATGTTG	50.0	50.0	57.0	57.7	232 bp	1.0	70.7
ycf2_p5	CCCAGCATTCATCAAGTTGG	GGATTTCCAAACCAACCCCA	50.0	50.0	57.0	58.3	238 bp	1.0	70.5