Hotspot

rps3

Genome · 84289-84981 · 693 bp

Back to Taxodium

Pi 0.00289

MarkerSeek score 60.40

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 693
variable_sites: 2
pi: 0.00289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	ACATTGGAGGATGTAACTGACTC	GCATAGCACTGCTCCAAGAT	43.5	50.0	57.9	58.0	899 bp	1.0	55.2
rps3_p2	ACATTGGAGGATGTAACTGACTC	CTCAACGAGTCGCACACTAA	43.5	50.0	57.9	58.0	919 bp	1.0	55.1
rps3_p3	ACATTGGAGGATGTAACTGACTC	TCAACGAGTCGCACACTAAG	43.5	50.0	57.9	58.0	918 bp	1.0	55.1
rps3_p4	TGGAGGATGTAACTGACTCT	GCATAGCACTGCTCCAAGAT	45.0	50.0	54.9	58.0	895 bp	1.0	54.9
rps3_p5	ACATTGGAGGATGTAACTGACTC	AGTCGCACACTAAGCATAGC	43.5	50.0	57.9	58.1	912 bp	1.0	54.9