Hotspot

ndhK

Genome · 126901-127707 · 807 bp

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Pi 0.00124

MarkerSeek score 59.50

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: Genome
length_bp: 807
variable_sites: 1
pi: 0.00124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	GTCTTTCTCTATCCGTGGGC	TCCTACTCGTTACTTTTTACTAACG	55.0	36.0	57.8	56.7	941 bp	1.0	40.1
ndhK_p2	TTTCTCTATCCGTGGGCCAT	TCCTACTCGTTACTTTTTACTAACG	50.0	36.0	58.5	56.7	938 bp	1.0	40.1
ndhK_p3	GTCTTTCTCTATCCGTGGGC	CCTACTCGTTACTTTTTACTAACG	55.0	37.5	57.8	55.4	940 bp	1.0	40.1
ndhK_p4	GTCTTTCTCTATCCGTGGGC	TTCCTACTCGTTACTTTTTACTAACG	55.0	34.6	57.8	57.3	942 bp	1.0	40.1
ndhK_p5	TTTCTCTATCCGTGGGCCAT	CCTACTCGTTACTTTTTACTAACG	50.0	37.5	58.5	55.4	937 bp	1.0	40.1