Hotspot

trnV-UAC-ndhC

Genome · 125222-126568 · 1347 bp

Back to Taxodium

Pi 0.00297

MarkerSeek score 60.50

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: Genome
length_bp: 1347
variable_sites: 4
pi: 0.00297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	TGGGTTCTTGGAACAGTTCG	TTGAGAATGCCAGAATCGGG	50.0	50.0	58.0	58.0	1517-1526 bp	1.0	79.6
trnV-UAC-ndhC_p2	TGCATGTTGGGTTCTTGGAA	TTGAGAATGCCAGAATCGGG	45.0	50.0	57.9	58.0	1524-1533 bp	1.0	79.3
trnV-UAC-ndhC_p3	AGAAGGTCTACGGTTCGAGT	TTGAGAATGCCAGAATCGGG	50.0	50.0	57.8	58.0	1459-1468 bp	1.0	78.4
trnV-UAC-ndhC_p4	GGGTTCTTGGAACAGTTCGA	TTGAGAATGCCAGAATCGGG	50.0	50.0	57.8	58.0	1516-1525 bp	1.0	77.9
trnV-UAC-ndhC_p5	ATGCATGTTGGGTTCTTGGA	TTGAGAATGCCAGAATCGGG	45.0	50.0	57.7	58.0	1525-1534 bp	1.0	77.5