Hotspot

clpP-accD

Genome · 116266-117487 · 1222 bp

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Pi 0.00393

MarkerSeek score 62.50

Primer available yes

Label clpP-accD

Feature Metadata

label_name: clpP-accD
feature_type: igs
region: Genome
length_bp: 1222
variable_sites: 4
pi: 0.00393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-accD_p1	CCTTGATAGGGCACTTTCGG	CATGGTTCCACGAGCTCTTT	55.0	50.0	58.3	58.2	1132-1330 bp	1.0	80.1
clpP-accD_p2	AGTTTGCTTCCTCCTCTTCG	CATGGTTCCACGAGCTCTTT	50.0	50.0	57.5	58.2	1155-1353 bp	1.0	79.1
clpP-accD_p3	ATTCGACCCAGTTTGCTTCC	CATGGTTCCACGAGCTCTTT	50.0	50.0	58.5	58.2	1164-1362 bp	1.0	79.0
clpP-accD_p4	GTTTGCTTCCTCCTCTTCGG	CATGGTTCCACGAGCTCTTT	55.0	50.0	58.6	58.2	1154-1352 bp	1.0	78.3
clpP-accD_p5	CCTTGATAGGGCACTTTCGG	GATACCATGGTTCCACGAGC	55.0	55.0	58.3	58.4	1137-1335 bp	1.0	78.3