Hotspot

psbJ-clpP

Genome · 114706-115512 · 807 bp

Back to Taxodium

Pi 0.0013

MarkerSeek score 64.20

Primer available yes

Label psbJ-clpP

Feature Metadata

label_name: psbJ-clpP
feature_type: igs
region: Genome
length_bp: 807
variable_sites: 1
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-clpP_p1	CGGAAGGATCCCTCTTTGGT	CGTGATTTTGCTCGTGATCG	55.0	50.0	59.1	57.9	958-997 bp	1.0	74.1
psbJ-clpP_p2	GGAAGGATCCCTCTTTGGTT	CGTGATTTTGCTCGTGATCG	50.0	50.0	56.8	57.9	957-996 bp	1.0	73.1
psbJ-clpP_p3	CGGAAGGATCCCTCTTTGGT	CCTACGCGATCAAGTGCTTA	55.0	50.0	59.1	57.8	913-952 bp	1.0	73.1
psbJ-clpP_p4	GGAAGGATCCCTCTTTGGTTG	CGTGATTTTGCTCGTGATCG	52.4	50.0	58.3	57.9	957-996 bp	1.0	72.6
psbJ-clpP_p5	GGAAGGATCCCTCTTTGGTT	CCTACGCGATCAAGTGCTTA	50.0	50.0	56.8	57.8	912-951 bp	1.0	72.2