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ycf1-rps12

Genome · 108457-109232 · 776 bp

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Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label ycf1-rps12

Feature Metadata

label_name: ycf1-rps12
feature_type: igs
region: Genome
length_bp: 776
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps12_p1	ATACATCTGAGCTGGACCGA	TGGCACATACTCCTTTACGC	50.0	50.0	57.9	58.0	990 bp	1.0	79.3
ycf1-rps12_p2	TACATCTGAGCTGGACCGAT	TGGCACATACTCCTTTACGC	50.0	50.0	57.9	58.0	989 bp	1.0	79.3
ycf1-rps12_p3	AAACAGCACAATCGGAATGC	TGGCACATACTCCTTTACGC	45.0	50.0	57.6	58.0	936 bp	1.0	77.1
ycf1-rps12_p4	ATACATCTGAGCTGGACCGA	GGCACATACTCCTTTACGCT	50.0	50.0	57.9	57.7	989 bp	1.0	77.0
ycf1-rps12_p5	TACATCTGAGCTGGACCGAT	GGCACATACTCCTTTACGCT	50.0	50.0	57.9	57.7	988 bp	1.0	77.0