Hotspot

rps19-rpl22

Genome · 83739-83829 · 91 bp

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Pi 0.0111

MarkerSeek score 59.90

Primer available yes

Label rps19-rpl22

Feature Metadata

label_name: rps19-rpl22
feature_type: igs
region: Genome
length_bp: 91
variable_sites: 1
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl22_p1	TTCCAGGGACATGCGAAAAA	AACCACGAAGTTGATGAGCT	45.0	45.0	58.0	57.4	259-260 bp	1.0	76.1
rps19-rpl22_p2	TTTCCAGGGACATGCGAAAA	AACCACGAAGTTGATGAGCT	45.0	45.0	58.0	57.4	260-261 bp	1.0	76.1
rps19-rpl22_p3	TTTTCCAGGGACATGCGAAA	AACCACGAAGTTGATGAGCT	45.0	45.0	58.0	57.4	261-262 bp	1.0	76.1
rps19-rpl22_p4	TCCATTTTCCAGGGACATGC	AACCACGAAGTTGATGAGCT	50.0	45.0	58.1	57.4	265-266 bp	1.0	75.0
rps19-rpl22_p5	ATTTTCCAGGGACATGCGAA	AACCACGAAGTTGATGAGCT	45.0	45.0	57.8	57.4	262-263 bp	1.0	74.5