Hotspot

trnI-CAU-ycf2

Genome · 73242-73951 · 710 bp

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Pi 0

MarkerSeek score 60.70

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: Genome
length_bp: 710
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	CCGCGACTTCCCAATTATGA	TAGACCACGGGTTCTCATGT	50.0	50.0	58.1	58.1	702-790 bp	1.0	83.0
trnI-CAU-ycf2_p2	CCGCGACTTCCCAATTATGA	GACCACGGGTTCTCATGTTT	50.0	50.0	58.1	58.1	700-788 bp	1.0	82.7
trnI-CAU-ycf2_p3	CCGCGACTTCCCAATTATGA	GTAGACCACGGGTTCTCATG	50.0	55.0	58.1	57.7	703-791 bp	1.0	81.2
trnI-CAU-ycf2_p4	CCGCGACTTCCCAATTATGA	ACCACGGGTTCTCATGTTTT	50.0	45.0	58.1	57.6	699-787 bp	1.0	80.7
trnI-CAU-ycf2_p5	GGTGCTTTAACCATTCAGCC	TAGACCACGGGTTCTCATGT	50.0	50.0	57.3	58.1	678-766 bp	1.0	78.2