Hotspot

ndhF-trnN-GUU

Genome · 58391-59220 · 830 bp

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Pi 0.00122

MarkerSeek score 60.40

Primer available yes

Label ndhF-trnN-GUU

Feature Metadata

label_name: ndhF-trnN-GUU
feature_type: igs
region: Genome
length_bp: 830
variable_sites: 1
pi: 0.00122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnN-GUU_p1	ATGAGATGCTTAGGAGGGGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	58.3	57.7	995-1003 bp	1.0	76.2
ndhF-trnN-GUU_p2	GCTTAGGAGGGGGACGTATA	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.7	57.7	988-996 bp	1.0	75.8
ndhF-trnN-GUU_p3	ATGAGATGCTTAGGAGGGGG	CCGATTGGTCGTAGGTTCAA	55.0	50.0	58.3	57.6	957-965 bp	1.0	75.7
ndhF-trnN-GUU_p4	GCTTAGGAGGGGGACGTATA	CCGATTGGTCGTAGGTTCAA	55.0	50.0	57.7	57.6	950-958 bp	1.0	75.4
ndhF-trnN-GUU_p5	AGGAGAGGGAATGAGATGCT	TTCCTCAGTAGCTCAGTGGT	50.0	50.0	57.5	57.7	1005-1013 bp	1.0	74.3