Hotspot

rpl32-ndhF

Genome · 55515-56173 · 659 bp

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Pi 0.00152

MarkerSeek score 59.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: Genome
length_bp: 659
variable_sites: 1
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TTTTGCTGCGGCCCAATA	GGAAGGAAAGGCACAATCCA	50.0	50.0	57.9	58.1	793 bp	1.0	62.7
rpl32-ndhF_p2	CTTTTGCTGCGGCCCAATA	GGAAGGAAAGGCACAATCCA	52.6	50.0	59.1	58.1	794 bp	1.0	62.6
rpl32-ndhF_p3	TTTTGCTGCGGCCCAATA	AGGAGCCTAATCCGATTGGT	50.0	50.0	57.9	58.2	828 bp	1.0	61.7
rpl32-ndhF_p4	CTTTTGCTGCGGCCCAATA	AGGAGCCTAATCCGATTGGT	52.6	50.0	59.1	58.2	829 bp	1.0	61.7
rpl32-ndhF_p5	TTTTGCTGCGGCCCAATA	GGCGAACACTCTTAGTTGCT	50.0	50.0	57.9	58.2	861 bp	1.0	61.6