Hotspot

atpI-atpH

Genome · 38220-38874 · 655 bp

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Pi 0.0046

MarkerSeek score 61.80

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 655
variable_sites: 3
pi: 0.0046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CGAATCCATGGAGGGTCATC	CCTACGGATAATCCAGCAGC	55.0	55.0	57.8	57.9	730-733 bp	1.0	78.6
atpI-atpH_p2	CGAATCCATGGAGGGTCATC	GCCTACGGATAATCCAGCAG	55.0	55.0	57.8	57.9	731-734 bp	1.0	78.6
atpI-atpH_p3	CGAATCCATGGAGGGTCATC	AACGGAAGCAGCAGAAATCA	55.0	45.0	57.8	58.1	707-710 bp	1.0	78.4
atpI-atpH_p4	CGAATCCATGGAGGGTCATC	ACGGAAGCAGCAGAAATCAA	55.0	45.0	57.8	58.1	706-709 bp	1.0	78.4
atpI-atpH_p5	CGAATCCATGGAGGGTCATC	GCCAGGTCCAATAGAAGCAA	55.0	50.0	57.8	57.9	752-755 bp	1.0	78.2