Hotspot

petN-trnC-GCA

Genome · 25682-25992 · 311 bp

Back to Taxodium

Pi 0.00322

MarkerSeek score 46.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 311
variable_sites: 1
pi: 0.00322
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TAAAGCCCACTTCTACCCCA	GCGACATAGCCAAGTGGTAA	50.0	50.0	58.0	58.0	470 bp	1.0	79.6
petN-trnC-GCA_p2	ACTACCATTAGAGCAGCCCA	GCGACATAGCCAAGTGGTAA	50.0	50.0	58.1	58.0	422 bp	1.0	79.1
petN-trnC-GCA_p3	TAAAGCCCACTTCTACCCCA	AATCCTCGATCCCCAGTTCA	50.0	50.0	58.0	58.1	436 bp	1.0	78.8
petN-trnC-GCA_p4	TAAAGCCCACTTCTACCCCA	ATCCTCGATCCCCAGTTCAA	50.0	50.0	58.0	58.1	435 bp	1.0	78.8
petN-trnC-GCA_p5	ACTACCATTAGAGCAGCCCA	ATCCTCGATCCCCAGTTCAA	50.0	50.0	58.1	58.1	387 bp	1.0	78.3