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trnD-GUC-psbM

Genome · 23015-24751 · 1737 bp

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Pi 0.0023

MarkerSeek score 59.60

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: Genome
length_bp: 1737
variable_sites: 4
pi: 0.0023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTAGAGTACCGCCCTGTCAA	GCTTGACTCGCCGTTTTTAC	50.0	50.0	58.1	58.1	1890-1891 bp	1.0	79.2
trnD-GUC-psbM_p2	ATTGGTTAGAGTACCGCCCT	GCTTGACTCGCCGTTTTTAC	50.0	50.0	58.1	58.1	1895-1896 bp	1.0	78.7
trnD-GUC-psbM_p3	TTAGAGTACCGCCCTGTCAA	CTTGACTCGCCGTTTTTACG	50.0	50.0	58.1	57.8	1889-1890 bp	1.0	77.9
trnD-GUC-psbM_p4	ATTGGTTAGAGTACCGCCCT	CTTGACTCGCCGTTTTTACG	50.0	50.0	58.1	57.8	1894-1895 bp	1.0	77.5
trnD-GUC-psbM_p5	TTAGAGTACCGCCCTGTCAA	CTGTACTTCGGCTTGACTCG	50.0	55.0	58.1	58.4	1900-1901 bp	1.0	76.5