Hotspot

ycf1

SSC · 95369-100378 · 5010 bp

Back to Taxillus

Pi 0.0245

MarkerSeek score 66.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5010
variable_sites: 492
pi: 0.0245
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTGACAAGGAAGATAAGCCA	CTTTTGGTCCTCCCCGAAAA	42.9	50.0	56.9	58.0	766-844 bp	0.928571	72.5
ycf1_p2	TGTGACAAGGAAGATAAGCCA	TTTGGTCCTCCCCGAAAAAG	42.9	50.0	56.9	58.0	764-842 bp	0.928571	72.5
ycf1_p3	TGTGACAAGGAAGATAAGCCA	AGTTTGGGGAATGGAAGCTG	42.9	50.0	56.9	58.1	792-870 bp	0.928571	72.0
ycf1_p4	TGTGACAAGGAAGATAAGCCA	CAGTTTGGGGAATGGAAGCT	42.9	50.0	56.9	58.1	793-871 bp	0.928571	72.0
ycf1_p5	TGTGACAAGGAAGATAAGCCA	AATTTCCCGAATGGTCCGAG	42.9	50.0	56.9	58.0	1066-1069 bp	0.214286	32.0