Hotspot

accD-psaI

LSC · 47141-47694 · 554 bp

Back to Taxillus

Pi 0.0525

MarkerSeek score 81.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 107
pi: 0.0525
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACAGGATTACCGGAAGCTG	GCTGGAATTACTAGGCCCAC	55.0	55.0	58.1	58.0	1100-1176 bp	1.0	91.3
accD-psaI_p2	GACAGGATTACCGGAAGCTG	GCCATTGCAAATGCTGGAAT	55.0	45.0	58.1	58.0	1112-1188 bp	1.0	91.3
accD-psaI_p3	ACCCGTTTGATCGAGTATGC	GCTGGAATTACTAGGCCCAC	50.0	55.0	58.1	58.0	976-1052 bp	1.0	91.2
accD-psaI_p4	ACCCGTTTGATCGAGTATGC	GCCATTGCAAATGCTGGAAT	50.0	45.0	58.1	58.0	988-1064 bp	1.0	91.2
accD-psaI_p5	CTGAGCTTGATGCAAATGGC	GCTGGAATTACTAGGCCCAC	50.0	55.0	58.1	58.0	889-965 bp	1.0	91.1