Hotspot

trnF-GAA-trnM-CAU

LSC · 40465-40901 · 437 bp

Back to Taxillus

Pi 0.0485

MarkerSeek score 66.60

Primer available yes

Label trnF-GAA-trnM-CAU

Feature Metadata

label_name: trnF-GAA-trnM-CAU
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 70
pi: 0.0485
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnM-CAU_p1	GGGGATATGGCGAAATTGGT	ACTTGAGGAAAGCGAAAGGG	50.0	50.0	58.0	58.1	1100-1481 bp	1.0	90.8
trnF-GAA-trnM-CAU_p2	CCTCGTGTCACCAGTTCAAA	AAGTTACCGCGAAGGCTATG	50.0	50.0	58.1	58.1	938-1228 bp	1.0	90.2
trnF-GAA-trnM-CAU_p3	GGGGATATGGCGAAATTGGT	AAGTTACCGCGAAGGCTATG	50.0	50.0	58.0	58.1	1453-1834 bp	1.0	90.0
trnF-GAA-trnM-CAU_p4	GGGGATATGGCGAAATTGGT	GTTACCGCGAAGGCTATGAA	50.0	50.0	58.0	58.1	1451-1832 bp	1.0	90.0
trnF-GAA-trnM-CAU_p5	GGGGATATGGCGAAATTGGT	TTACCGCGAAGGCTATGAAC	50.0	50.0	58.0	58.1	1450-1831 bp	1.0	90.0