Hotspot

psaA-ycf3

LSC · 34730-35329 · 600 bp

Back to Taxillus

Pi 0.0385

MarkerSeek score 93.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 600
variable_sites: 77
pi: 0.0385
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 93.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	720-779 bp	1.0	90.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	707-766 bp	1.0	89.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	711-770 bp	1.0	89.5
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	706-765 bp	1.0	88.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGTTTCGAATAA	50.0	45.0	57.9	57.6	700-759 bp	1.0	88.3