Hotspot

trnT-GGU-psbD

LSC · 24929-25856 · 928 bp

Back to Taxillus

Pi 0.03

MarkerSeek score 75.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 109
pi: 0.03
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CGGATTTGAACCAGCGTAGA	GGCCCCAGAGTAACAACAAA	50.0	50.0	58.0	58.0	1678-2011 bp	1.0	91.6
trnT-GGU-psbD_p2	ACAGAGACCCCTTTCGGTAA	GGCCCCAGAGTAACAACAAA	50.0	50.0	58.0	58.0	1291-1620 bp	1.0	91.6
trnT-GGU-psbD_p3	CGGATTTGAACCAGCGTAGA	AGCTTGGGTGGGGTTAAAAG	50.0	50.0	58.0	58.0	2115-2448 bp	1.0	90.3
trnT-GGU-psbD_p4	CGGATTTGAACCAGCGTAGA	AGTTTCTTCAGCTTGGGTGG	50.0	50.0	58.0	58.0	2124-2457 bp	1.0	90.2
trnT-GGU-psbD_p5	ACAGAGACCCCTTTCGGTAA	AGCTTGGGTGGGGTTAAAAG	50.0	50.0	58.0	58.0	1728-2057 bp	1.0	90.0