Hotspot

petN-psbM

LSC · 22633-23412 · 780 bp

Back to Taxillus

Pi 0.0285

MarkerSeek score 77.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 97
pi: 0.0285
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATCGCTTGGGCTGCTTTAAT	ACGGCTAGTCAAAATGATTTTTCA	45.0	33.3	58.2	57.7	680-934 bp	1.0	55.5
petN-psbM_p2	GTATCGCTTGGGCTGCTTTA	ACGGCTAGTCAAAATGATTTTTCA	50.0	33.3	58.3	57.7	682-936 bp	1.0	54.7
petN-psbM_p3	TATCGCTTGGGCTGCTTTAA	ACGGCTAGTCAAAATGATTTTTCA	45.0	33.3	57.6	57.7	681-935 bp	1.0	54.1
petN-psbM_p4	ATCGCTTGGGCTGCTTTAAT	AAAACGGCTAGTCAAAATGA	45.0	35.0	58.2	53.4	783-937 bp	0.928571	50.6
petN-psbM_p5	ATCGCTTGGGCTGCTTTAAT	TGTAAAAACGGCTAGTCAAAATGA	45.0	33.3	58.2	57.4	787-941 bp	0.928571	50.3