Hotspot

rpoB-trnC-GCA

LSC · 21497-22181 · 685 bp

Back to Taxillus

Pi 0.0564

MarkerSeek score 86.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 118
pi: 0.0564
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATCCCAGAGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	724-835 bp	0.928571	88.3
rpoB-trnC-GCA_p2	CCTCATTTCCATCCCAGAGC	AACTGGGGAAAGGGGATTTG	55.0	50.0	58.0	57.7	715-826 bp	0.928571	86.4
rpoB-trnC-GCA_p3	CCTCATTTCCATCCCAGAGC	GGGGATTTCGGGTCTTTTGT	55.0	50.0	58.0	58.1	758-869 bp	0.857143	85.5
rpoB-trnC-GCA_p4	CCTCATTTCCATCCCAGAGC	ACAGAATTGGGGGATTTCGG	55.0	50.0	58.0	57.9	767-878 bp	0.857143	85.0
rpoB-trnC-GCA_p5	CCTCATTTCCATCCCAGAGC	ATTTGAACTGGGGAAAGGGG	55.0	50.0	58.0	57.7	720-818 bp	0.857143	83.5