Hotspot

trnS-GCU-trnR-UCU

LSC · 4785-5248 · 464 bp

Back to Taxillus

Pi 0.0564

MarkerSeek score 78.50

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 104
pi: 0.0564
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGCCGCTTTA	TGTGGGCATTTCTGTTTCGA	45.0	45.0	58.0	58.0	1111-1266 bp	1.0	91.5
trnS-GCU-trnR-UCU_p2	CGCATAACTCGTACAACGGA	TGTGGGCATTTCTGTTTCGA	50.0	45.0	58.1	58.0	1130-1285 bp	1.0	91.0
trnS-GCU-trnR-UCU_p3	ATTAGCAATCCGCCGCTTTA	GTTTCGAGAGTAGGATCCGC	45.0	55.0	58.0	57.9	1098-1253 bp	1.0	90.7
trnS-GCU-trnR-UCU_p4	ATTAGCAATCCGCCGCTTTA	CCCGCTATTAATGTGGGCAT	45.0	50.0	58.0	58.1	1122-1265 bp	0.785714	80.4
trnS-GCU-trnR-UCU_p5	CGCATAACTCGTACAACGGA	CCCGCTATTAATGTGGGCAT	50.0	50.0	58.1	58.1	1141-1284 bp	0.785714	79.8