Hotspot

trnH-GUG-psbA

LSC · 46-186 · 141 bp

Back to Taxillus

Pi 0.0465
MarkerSeek score 81.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
141
variable_sites
19
pi
0.0465
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 254-340 bp 1.0 85.6
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 253-339 bp 1.0 85.6
trnH-GUG-psbA_p3 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 255-341 bp 1.0 85.5
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 255-341 bp 1.0 85.1
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 254-340 bp 1.0 85.1