Hotspot

ycf1

SSC · 125726-131338 · 5613 bp

Back to Tashiroea

Pi 0.0093

MarkerSeek score 49.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5613
variable_sites: 119
pi: 0.0093
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGGCCAGGATTATCCATGG	AGTCCGAGAATCACTACACAAC	50.0	45.5	57.3	57.8	1151-1202 bp	1.0	62.7
ycf1_p2	TTGGCCAGGATTATCCATGG	GTCCGAGAATCACTACACAACT	50.0	45.5	57.3	57.8	1150-1201 bp	1.0	62.7
ycf1_p3	TTGGCCAGGATTATCCATGG	CATCCCTTACCATTCCCATTCA	50.0	45.5	57.3	57.8	1486-1537 bp	1.0	62.0
ycf1_p4	TTGGCCAGGATTATCCATGG	TCCCTTACCATTCCCATTCA	50.0	45.0	57.3	55.8	1484-1535 bp	1.0	61.7
ycf1_p5	TTGGCCAGGATTATCCATGG	TCTGGCTGATAGAACAAACACA	50.0	40.9	57.3	57.6	1416-1467 bp	1.0	60.9