Hotspot

psbA

LSC · 305-1366 · 1062 bp

Back to Tashiroea

Pi 0.00408

MarkerSeek score 40.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 10
pi: 0.00408
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	AACACAAAACTACTGGCCGT	55.0	45.0	58.1	58.0	2046-2049 bp	1.0	81.1
psbA_p2	GATCCACTTGGCTACATCCG	ACAAAACTACTGGCCGTGTT	55.0	45.0	58.1	58.0	2043-2046 bp	1.0	81.1
psbA_p3	CAATCCACTGCCTTGATCCA	AACACAAAACTACTGGCCGT	50.0	45.0	57.9	58.0	2060-2063 bp	1.0	81.0
psbA_p4	CAATCCACTGCCTTGATCCA	ACAAAACTACTGGCCGTGTT	50.0	45.0	57.9	58.0	2057-2060 bp	1.0	81.0
psbA_p5	AATCCACTGCCTTGATCCAC	ACAAAACTACTGGCCGTGTT	50.0	45.0	57.9	58.0	2056-2059 bp	1.0	81.0