Hotspot

ndhF

SSC · 112427-114670 · 2244 bp

Back to Tashiroea

Pi 0.0103
MarkerSeek score 58.30
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2244
variable_sites
55
pi
0.0103
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TCCAAAATGTCAATCCAGAGT TCGAAGGAATTGAGAAAGAA 38.1 35.0 55.1 52.1 2327-2344 bp 0.833333 35.4
ndhF_p2 TCCAAAATGTCAATCCAGAGT TTCGAAGGAATTGAGAAAGA 38.1 35.0 55.1 52.1 2328-2345 bp 0.833333 35.4
ndhF_p3 CATCCAAAATGTCAATCCAGAGT TCGAAGGAATTGAGAAAGAA 39.1 35.0 57.1 52.1 2329-2346 bp 0.833333 35.4
ndhF_p4 CATCCAAAATGTCAATCCAGAGT TTCGAAGGAATTGAGAAAGA 39.1 35.0 57.1 52.1 2330-2347 bp 0.833333 35.4
ndhF_p5 TCCAAAATGTCAATCCAGAGT CCTTCTTCCTATGAATATTCGAAGG 38.1 40.0 55.1 57.1 2345-2362 bp 0.833333 35.4