Hotspot

rps8-rpl14

LSC · 81826-82149 · 324 bp

Back to Tashiroea

Pi 0.0395

MarkerSeek score 66.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 25
pi: 0.0395
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATTACAATTCCCATCCCGCC	CGATTGCCCGAGAATTGAGA	50.0	50.0	58.0	58.1	639-712 bp	1.0	87.2
rps8-rpl14_p2	ATTACAATTCCCATCCCGCC	CTTAAACGCAACAACGGCAT	50.0	45.0	58.0	58.0	736-809 bp	1.0	86.9
rps8-rpl14_p3	ATTACAATTCCCATCCCGCC	CACGTATGGGTTCAGGGAAG	50.0	55.0	58.0	58.0	1253-1329 bp	1.0	86.0
rps8-rpl14_p4	CCCAATTCCTTCCAGTCGAG	CACGTATGGGTTCAGGGAAG	55.0	55.0	58.0	58.0	1311-1387 bp	1.0	85.7
rps8-rpl14_p5	ATTACAATTCCCATCCCGCC	CCTACGGAAACACGTATGGG	50.0	55.0	58.0	58.1	1263-1339 bp	1.0	85.6