Hotspot

psbT-psbN

LSC · 75591-75662 · 72 bp

Back to Tashiroea

Pi 0.0565

MarkerSeek score 62.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 11
pi: 0.0565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AGTTCCAACTAAAAAGATGAAATGA	TTCGAAGAACACGGGGACTA	28.0	50.0	55.4	58.4	118 bp	1.0	47.5
psbT-psbN_p2	AGTTCCAACTAAAAAGATGAAATGA	ATCCATTCGAAGAACACGGG	28.0	50.0	55.4	58.0	123 bp	1.0	47.2
psbT-psbN_p3	GTTCCAACTAAAAAGATGAAATGA	ATCCATTCGAAGAACACGGG	29.2	50.0	54.1	58.0	122 bp	1.0	47.2
psbT-psbN_p4	AGTTCCAACTAAAAAGATGAAATG	ATCCATTCGAAGAACACGGG	29.2	50.0	54.0	58.0	123 bp	1.0	47.2
psbT-psbN_p5	AAGTTCCAACTAAAAAGATGAAATGA	ATCCATTCGAAGAACACGGG	26.9	50.0	56.1	58.0	124 bp	1.0	47.1