Hotspot

psbE-petL

LSC · 65715-66845 · 1131 bp

Back to Tashiroea

Pi 0.0103

MarkerSeek score 57.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1131
variable_sites: 27
pi: 0.0103
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	57.8	57.6	1198-1258 bp	1.0	54.9
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GCTGAGGTTATAGTTAAAGCTGC	55.0	43.5	58.2	57.6	1193-1253 bp	1.0	54.6
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.4	57.6	1191-1251 bp	1.0	53.3
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCTGCTAGTAGAAAACCA	50.0	40.0	57.8	54.3	1182-1242 bp	1.0	52.5
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCAA	50.0	40.0	57.8	54.3	1181-1241 bp	1.0	52.5