Hotspot

ndhC-trnV-UAC

LSC · 51799-52407 · 609 bp

Back to Tashiroea

Pi 0.0203

MarkerSeek score 72.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 609
variable_sites: 27
pi: 0.0203
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGACCTTTACTAATCGGGGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.7	57.9	744-863 bp	1.0	81.8
ndhC-trnV-UAC_p2	AATCGGGGCTAAAATTCCGG	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	733-852 bp	1.0	81.3
ndhC-trnV-UAC_p3	GGACCTTTACTAATCGGGGC	CGGTTCGAATCCGTATAGCC	55.0	55.0	57.7	58.3	745-864 bp	1.0	79.9
ndhC-trnV-UAC_p4	CGGGGCTAAAATTCCGGAAA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.5	57.9	730-849 bp	1.0	79.6
ndhC-trnV-UAC_p5	AATCGGGGCTAAAATTCCGG	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	734-853 bp	1.0	79.5