Hotspot

trnC-GCA-ndhJ

LSC · 49190-50076 · 887 bp

Back to Tashiroea

Pi 0.0158

MarkerSeek score 53.50

Primer available yes

Label trnC-GCA-ndhJ

Feature Metadata

label_name: trnC-GCA-ndhJ
feature_type: igs
region: LSC
length_bp: 887
variable_sites: 32
pi: 0.0158
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	935-943 bp	1.0	44.2
trnC-GCA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTGAAATACAAGATGCTCATTGA	50.0	30.4	58.1	54.6	936-944 bp	1.0	44.2
trnC-GCA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	TGAAATACAAGATGCTCATTGA	55.0	31.8	57.9	53.8	959-967 bp	1.0	44.1
trnC-GCA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	TGAAATACAAGATGCTCATTGA	55.0	31.8	58.0	53.8	971-979 bp	1.0	44.0
trnC-GCA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TTGAAATACAAGATGCTCATTGA	55.0	30.4	58.0	54.6	972-980 bp	1.0	44.0